Western Blot:Article Title: In mice and humans, brain microvascular contractility matures postnatally Brain microvessel post-natal maturation
Article Snippet: .. Resources table Page 10/26 Reagent or resource Source Reference Antibodies SMA (WB : 1/1000, IF : 1/250) Sigma C6198 MYH11 (IF / human slices: 1/50) Sigma HPA015310-100UL Myh11 (Western Blot 1/1000) Abcam ab53219 Pecam-1 (IF: 1/300) R&d systems AF3628 Histone 3 (Western Blot: 1/2000) Ozyme 14269S anti-goat, alexa 647 (IF : 1/2000) Thermo sher anti-rabbit, HRP (WB : 1/2500) Cohesion CSA2115 anti-mouse, HRP (WB : 1/2500) Cohesion CSA2108 Alexa-conjugated Isolectin (griffonia simplicifolia) Thermo sher I32450 FISH Probes Myh11 ACD/Biotechne 316101 qPCR Primers Tagln Forward CCCAGACACCGAAGCTACTC Reverse TCGATCCCTCAGGATACAGG Sigma Acta2 Forward GTCCCAGACATCAGGGAGTAA Reverse TCGGATACTTCAGCGTCAGGA Sigma Myh11 Forward AACGCCCTCAAGAGCAAACTCAGA Reverse TCCCGAGCGTCCATTTCTTCTTCA Sigma Atp1b1 Forward GCTGCTAACCATCAGTGAACT Reverse GGGGTCATTAGGACGGAAGGA Sigma Gapdh Forward AGGTCGGTGTGAACGGATTTG Reverse TGTAGACCATGTAGTTGAGGTCA Sigma Page 11/26 Tbxar2 Forward CCTTGTTCTCACCGACTTCC Reverse GCTGAACCATCATCTCCACC Sigma qPCR probes Kcnj8 Thermo sher Mm00438070_m1 Abcc9 Thermo sher Mm00447761_m1 Pecam1 Thermo sher Mm00487656_m1 Gapdh Thermo sher Mm00501337_m1 Software and Algorithms ImageJ https://imagej.nih.gov/ij/ QuPath https://qupath.github.io/ Deposited Data Raw data and analysis This paper GEO: GSE173844 Results MVs follow a transcriptional maturation program between P5 and P15 Several studies in rodents have suggested that the brain’s microvascular system architecture reorganizes after birth (Coelho-Santos et al. 2021; Coelho-Santos and Shih 2020). ..
Fluorescence In Situ Hybridization:Article Title: In mice and humans, brain microvascular contractility matures postnatally Brain microvessel post-natal maturation
Article Snippet: .. Resources table Page 10/26 Reagent or resource Source Reference Antibodies SMA (WB : 1/1000, IF : 1/250) Sigma C6198 MYH11 (IF / human slices: 1/50) Sigma HPA015310-100UL Myh11 (Western Blot 1/1000) Abcam ab53219 Pecam-1 (IF: 1/300) R&d systems AF3628 Histone 3 (Western Blot: 1/2000) Ozyme 14269S anti-goat, alexa 647 (IF : 1/2000) Thermo sher anti-rabbit, HRP (WB : 1/2500) Cohesion CSA2115 anti-mouse, HRP (WB : 1/2500) Cohesion CSA2108 Alexa-conjugated Isolectin (griffonia simplicifolia) Thermo sher I32450 FISH Probes Myh11 ACD/Biotechne 316101 qPCR Primers Tagln Forward CCCAGACACCGAAGCTACTC Reverse TCGATCCCTCAGGATACAGG Sigma Acta2 Forward GTCCCAGACATCAGGGAGTAA Reverse TCGGATACTTCAGCGTCAGGA Sigma Myh11 Forward AACGCCCTCAAGAGCAAACTCAGA Reverse TCCCGAGCGTCCATTTCTTCTTCA Sigma Atp1b1 Forward GCTGCTAACCATCAGTGAACT Reverse GGGGTCATTAGGACGGAAGGA Sigma Gapdh Forward AGGTCGGTGTGAACGGATTTG Reverse TGTAGACCATGTAGTTGAGGTCA Sigma Page 11/26 Tbxar2 Forward CCTTGTTCTCACCGACTTCC Reverse GCTGAACCATCATCTCCACC Sigma qPCR probes Kcnj8 Thermo sher Mm00438070_m1 Abcc9 Thermo sher Mm00447761_m1 Pecam1 Thermo sher Mm00487656_m1 Gapdh Thermo sher Mm00501337_m1 Software and Algorithms ImageJ https://imagej.nih.gov/ij/ QuPath https://qupath.github.io/ Deposited Data Raw data and analysis This paper GEO: GSE173844 Results MVs follow a transcriptional maturation program between P5 and P15 Several studies in rodents have suggested that the brain’s microvascular system architecture reorganizes after birth (Coelho-Santos et al. 2021; Coelho-Santos and Shih 2020). ..
Real-time Polymerase Chain Reaction:Article Title: In mice and humans, brain microvascular contractility matures postnatally Brain microvessel post-natal maturation
Article Snippet: .. Resources table Page 10/26 Reagent or resource Source Reference Antibodies SMA (WB : 1/1000, IF : 1/250) Sigma C6198 MYH11 (IF / human slices: 1/50) Sigma HPA015310-100UL Myh11 (Western Blot 1/1000) Abcam ab53219 Pecam-1 (IF: 1/300) R&d systems AF3628 Histone 3 (Western Blot: 1/2000) Ozyme 14269S anti-goat, alexa 647 (IF : 1/2000) Thermo sher anti-rabbit, HRP (WB : 1/2500) Cohesion CSA2115 anti-mouse, HRP (WB : 1/2500) Cohesion CSA2108 Alexa-conjugated Isolectin (griffonia simplicifolia) Thermo sher I32450 FISH Probes Myh11 ACD/Biotechne 316101 qPCR Primers Tagln Forward CCCAGACACCGAAGCTACTC Reverse TCGATCCCTCAGGATACAGG Sigma Acta2 Forward GTCCCAGACATCAGGGAGTAA Reverse TCGGATACTTCAGCGTCAGGA Sigma Myh11 Forward AACGCCCTCAAGAGCAAACTCAGA Reverse TCCCGAGCGTCCATTTCTTCTTCA Sigma Atp1b1 Forward GCTGCTAACCATCAGTGAACT Reverse GGGGTCATTAGGACGGAAGGA Sigma Gapdh Forward AGGTCGGTGTGAACGGATTTG Reverse TGTAGACCATGTAGTTGAGGTCA Sigma Page 11/26 Tbxar2 Forward CCTTGTTCTCACCGACTTCC Reverse GCTGAACCATCATCTCCACC Sigma qPCR probes Kcnj8 Thermo sher Mm00438070_m1 Abcc9 Thermo sher Mm00447761_m1 Pecam1 Thermo sher Mm00487656_m1 Gapdh Thermo sher Mm00501337_m1 Software and Algorithms ImageJ https://imagej.nih.gov/ij/ QuPath https://qupath.github.io/ Deposited Data Raw data and analysis This paper GEO: GSE173844 Results MVs follow a transcriptional maturation program between P5 and P15 Several studies in rodents have suggested that the brain’s microvascular system architecture reorganizes after birth (Coelho-Santos et al. 2021; Coelho-Santos and Shih 2020). ..
Software:Article Title: In mice and humans, brain microvascular contractility matures postnatally Brain microvessel post-natal maturation
Article Snippet: .. Resources table Page 10/26 Reagent or resource Source Reference Antibodies SMA (WB : 1/1000, IF : 1/250) Sigma C6198 MYH11 (IF / human slices: 1/50) Sigma HPA015310-100UL Myh11 (Western Blot 1/1000) Abcam ab53219 Pecam-1 (IF: 1/300) R&d systems AF3628 Histone 3 (Western Blot: 1/2000) Ozyme 14269S anti-goat, alexa 647 (IF : 1/2000) Thermo sher anti-rabbit, HRP (WB : 1/2500) Cohesion CSA2115 anti-mouse, HRP (WB : 1/2500) Cohesion CSA2108 Alexa-conjugated Isolectin (griffonia simplicifolia) Thermo sher I32450 FISH Probes Myh11 ACD/Biotechne 316101 qPCR Primers Tagln Forward CCCAGACACCGAAGCTACTC Reverse TCGATCCCTCAGGATACAGG Sigma Acta2 Forward GTCCCAGACATCAGGGAGTAA Reverse TCGGATACTTCAGCGTCAGGA Sigma Myh11 Forward AACGCCCTCAAGAGCAAACTCAGA Reverse TCCCGAGCGTCCATTTCTTCTTCA Sigma Atp1b1 Forward GCTGCTAACCATCAGTGAACT Reverse GGGGTCATTAGGACGGAAGGA Sigma Gapdh Forward AGGTCGGTGTGAACGGATTTG Reverse TGTAGACCATGTAGTTGAGGTCA Sigma Page 11/26 Tbxar2 Forward CCTTGTTCTCACCGACTTCC Reverse GCTGAACCATCATCTCCACC Sigma qPCR probes Kcnj8 Thermo sher Mm00438070_m1 Abcc9 Thermo sher Mm00447761_m1 Pecam1 Thermo sher Mm00487656_m1 Gapdh Thermo sher Mm00501337_m1 Software and Algorithms ImageJ https://imagej.nih.gov/ij/ QuPath https://qupath.github.io/ Deposited Data Raw data and analysis This paper GEO: GSE173844 Results MVs follow a transcriptional maturation program between P5 and P15 Several studies in rodents have suggested that the brain’s microvascular system architecture reorganizes after birth (Coelho-Santos et al. 2021; Coelho-Santos and Shih 2020). ..
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